Ameliorative effect of bee products on in vitro maturation of sheep oocytes

The present study was undertaken to investigate the possible stimulating effects of Nigella sativa (N. sativa) honey, natural Saudi Sider and honeybee pollen in vitro maturation (IVM) medium sheep oocytes on their subsequent development. Hence, immature were exposed various concentrations sativa), honey (5, 10, 20%), as well (1, 50 μg/ml) during an period (24 hours). After exposure time, rate, glutathione (GSH) concentration, candidate gene expression (GDF-9, MPF, CMOS, IGF-1, BAX) evaluated. Our results showed that rate higher groups challenged with lowest level bee products (5% 1 when compared control group; where mean number metaphase II stage reached 0.360 for pollen-treated group, 0.293 N. sativa-treated 0.203 honey-treated group. significantly increased group other groups. Concerning results, treatment best all genes except CMOS gene, which than GI GII lower GIV BAX did not show a significant difference In conclusion, addition at low concentration IVM improved level, matured ovine oocytes.